How does a High-Performance Liquid Chromatograph - HPLC - Work

How does a High-Performance Liquid Chromatograph - HPLC - Work?

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 A RESERVOIR HOLDS THE SOLVENT:

Called the mobile phase, because it moves.

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* A HIGH-PRESSURE PUMP:

Solvent delivery system or solvent manager) is used to generate and meter a specified flow rate of mobile phase, typically milliliters per minute.
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* AN INJECTOR:

Sample manager “auto sampler” is able to introduce (inject) the sample into the
continuously flowing mobile phase stream that carries the sample into the HPLC
column.
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* THE COLUMN:

Contains the chromatographic packing material needed to effect the separation.
This packing material is called the stationary phase because it is held in place by the column hardware. Is needed to see the separated compound bands as they elute from the HPLC column (most compounds have no color, so we cannot see them with our eyes).
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* DETECTORS AND COMPUTER DATA STATION:

The detector is wired to the computer data station, the HPLC system component that records the electrical signal needed to generate the chromatogram on its display and to identify and quantities the concentration of the sample constituents.

** DETECTORS TYPES:
UV-absorbance detector -- fluorescence detector -- evaporative-light-scattering detector ,,, etc..